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Send Feedback. E-mail the story Flipping a light switch in the cell: Quantum dots used for targeted neural activation. Your friend's email. Your email. The best answers are voted up and rise to the top. Home Questions Tags Users Unanswered.
Ask Question. Asked 9 years, 2 months ago. Active 8 years ago. Viewed 3k times. Kamil Sindi. Kamil Sindi Kamil Sindi 1 1 gold badge 5 5 silver badges 12 12 bronze badges. Active Oldest Votes. Alan Rominger Alan Rominger Why do we have to keep the wavelength the same?
It's about signal-to-noise. Where can I find how one calculates the number of peaks seen? By resolvable peaks, do you mean having enough "particles" to create such peaks? Reading the Wikipedia for the double slit experiment might be the most helpful, although it doesn't address the practical size limit very well.
Anonymous Coward Anonymous Coward 1, 9 9 silver badges 8 8 bronze badges. Point taken on "size" comment. Thus, their corresponding antibodies are conjugated to particles to magnetically label these cells. Our company provides customized primary antibody conjugation service to assist you immobilizing your desired antibodies on magnetic particles.
Find our service at the link below: Immobilization of Antibody on Beads. Secondary antibodies have the ability to capture the primary antibodies. By pre-incubating cells with primary antibodies, cells of interest can be subsequently labeled with secondary antibody immobilized magnetic particles and thus be isolated from mixtures in the presence of a magnetic field. The streptavidin-biotin binding is the strongest known non-covalent interaction between a protein and ligand and has been exploited for use in many bioseparation processes.
A variety of biotinylated primary antibodies have been commercialized and readily available in the market. Current market offers a variety of magnetic particles with their size ranging from 50nm to several micrometers. A debated on which size range should be chosen for cell separation rises.
Some magnetic particle researchers insisted that nano-sized magnetic particles should be a proper choice for cell separation. They suggested that nano-sized particles offer a mild magnetic field force to separate cells, which causes low damage to the target cells. Meanwhile, nano-sized particles have been widely used and highly published.
On the other hand, another group of scholars argued that the nano-sized particles may be internalized by the cells during incubation. These internalized particles can enter the cell nuclei and influence cell biological processes, leading to an altered cell nature status and even cell death.
Whereas, micro-sized particles, since they are roughly the same size as mammalian cells, are less likely to be taken up by the cells. Thus, micro-sized particles should exhibit less influence on the nature status of cells.
Meantime, larger particles have a higher magnetic mobility and are capable of separating cells from viscous mixtures. Please do not hesitate to contact us if you need free consultation and a detailed quotation of your project.May 21, · The definitions of the seven base units of the SI, or the International System of Units, are now completely different. SIZE MATTERS The way we measured kilograms, meters, and seconds was all .